It is thus appropriate to modify as follows the existing Requirements for. Rabies Vaccine for Human Use. General considerations (page 58). Replace the. Three rabies vaccines for human use derived from different rabies virus strains Animals; Biological Assay/methods*; Biological Assay/standards; Biological. BIOLOGICAL ASSAY OF RABIES ANTISERUM: The potency of rabies antiserum is determined by comparing a lethal intracerebral dose of.

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Internal between two injection between minutes depending on the rate at which B. Similarly record the contraction of test preparation as standard.


Never has been confirmed Rabies virus never isolated from blood Rabies virus: Longitudinal Section biassay Rabies Virus. Automatically changes to Flash or non-Flash embed. Incubation time days. Viral disease – cause acute encephalitis inflammation of the brain in warm-blooded animals.

Bite from an infected animal. These two groups are used for titration of LD50 of challenge suspension. Sequential Steps of Rabies Transmission.

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Go to Application Have a question? On a graph paper, plot the transmittance value against the corresponding levels of std.

Then they are incubated or about 24 hours at about and the diameter or areas of the circular inhibition zones are measured. Peptone 6gm Pancreatic digest of casein 4gm Yeast extract 3gm Beaf extract 1. It is a neurosecretary product mainly synthesize in the cell bodies of paraventracular nuclear of the hypothalamus. The no of units per ml Either it is from horse of other mammals Recommended dose preservatives Slide 9: You do not have the permission to view this presentation.


Acid digested casein solution. Zoonotic animals to humans. Incubation at c for hrs. Observe the animals after 48hrs Mixture containing larger amount of toxin gives the larger reaction Mixture containing less amount of toxin gives the smaller reaction Slide 6: Freeze — dried preparation. Oxytocin may be presented as a solid or as a solution in a solvent containing an appropriate antimicrobial preservative such as 0.

The proposed fifth International Standard PISRAV which was derived from the same virus strain as the present international standard preparation, the Pitman Moore PM strain, was found to be approximately twice as potent relative to the International Standard in immunogenicity assays as in antigenicity assays.

Dose should be added at regular interval[minutes]. WordPress Embed Customize Embed. Dilute 25ml of basal medium stock solution with equal volume of H2O sterile in autoclave.

Prepare mixture of solution: Virus lifestyle Virus replicated in muscle cells near site of bite for most of incubation time. Test dose and std dose contains 0. Prepare fold dilution of std. Severity of exposure Location of the bite The biting animal Bites on head and neck have shorter incubation time as short as 15 days because of rich peripheral nerve supply Encephalitic Form: Rabies vaccine is a suspension of a suitable strain of fixed rabies grown in suitable approved cell culture and inactivated by a suitable method.

Oxytocin facilitates the contraction of uterus. Centrifuge decant of supernatant liquid [repeat 3 times].

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The vaccine is prepared immediately before use by reconstitution from the dried vaccine with a suitable sterile liquid. White mice weight 11gg. Dephtheria anti-toxin is preparation containing antitoxic globulins that they have the power of specifically neutralizing the toxin formed by Cornebacterium diphtheria.


Standard preparation containing freeze dried hyper immune horse serum. Standard preparation Test preparation To triplicate test tubes 0ml 0. Presentation Description No description available. Never has been confirmed Rabies virus never isolated from blood.

If it is derived from animal vaccinee. Consciousness initially spared Two forms can overlap or progress from one to the baccine Coma after one week of neuro symptoms with death a few days after. Presentation Description No description available. I am very happy to your post about on. Hallucinations, seizures, ataxia, focal weakness and arrhythmias can occur.

Animal Behavior Classic Picture of rabid, mangy dog foaming at the mouth…not often seen, signs frequently more subtle. The mixture of the preparation being examined that contains 0. The test toxin is allowed to stand for some months before being used for the assay of samples of antitoxin.

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Potency is determined by international reference. Any suitable strain rabies virus of known potency, such as the CVS strain may be used. The potency of rabies vaccine is determined by comparing a lethal intracerebral dose of a rabies virus with the dose of rabids standard preparation of rabies vaccines necessary to give for same protection.